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1mL Sample - Mogengel Matrix Gel for Organoid or 3-D Cell Culture

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Mogengelâ„¢ Matrix Gel, is a series of formular-optimized gel designed for diversified applications, including 2-D and 3-D cell culturing, cell proliferation, differentiation, and organoid culturing. The basement membrane matrix contains several components, components including laminin, collagen IV, entactin, and heparin sulfate proteoglycan, which were produced and purified from from gene-edited LDEV-free mouse tumor cells.

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Datasheet to download
[Data Sheet] Matrix Basic -082704
[Data Sheet] Matrix phenol red free-082706
[Data Sheet] Matrix GFR -082701
[Data Sheet] Matrix GFR phenol red free -082703
[Data Sheet] Matrix HC-082724
[Data Sheet] Matrix HC phenol red free-082726
[Data Sheet] Matrix HC GFR-082721
[Data Sheet]  Matrix HC GFR phenol red free-082723
[Data Sheet] Matrix IPSC Level-082777
[Data Sheet] Matrix Organoid Culture-082755

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Mogengel™ Matrix Gel forms continuous sheets of a specialized extracellular matrix which is an essential component in the organization of tissues forming, the scaffolding, supporting for cell growth and forming cell layers. The matrix gel can also affect cell function such as adhesion, migration, proliferation, and differentiation.

Advantages

>>> Validated by Cellular Function Assays
Strict application-based quality controls were established for every single product, including tumorigenesis, angiogenesis, and tumor cell invasion.

>>> Lot-to-Lot Consistency
Strict quality controls and quality management to ensure consistency of low level of endotoxin, viral, and bacterial contamination. 

>>> Stable, Long-term Supply
Large-scale manufacturing capacity paired with ACROBiosystems’ global supply chain to stabilize supply across the globe.

>>> Cost Effective Reagents
Bulk purchase pricing, organizational purchasing pricing and stable supply pricing plans to meet your various needs.

Mogengel™ Matrix Gel products undergo extensive quality tests to ensure its consistency in performance. Take advantage of BioBench’s global supply chain to receive it fast and worry-free!

Various BME Versions for Specific Applications
To better match your research applications, various formulars of the Matrix Gel are available! The series of products including phenol red-free versions, growth factor rich versions, high concentration versions, iPSC versions and organoid versions. Explore the various products for your specific application below to find the best match!

Product Name

Cat.

Size

General Application and Description

Matrix Gel (standard)

082704

0827045

082704T

10mL

5mL

1mL

2D or 3D cultures related to cell proliferation or differentiation,as well as study of cell morphological.

The commonly used experiments are on migration or invasion of tumor cells, in vitro angiogenesis, aortic ring and cell sphere culture. Protein concentration: 8-13mg/mL.

Matrix Gel (standard), Phenol Red Free

082706

0827065

082706T

10mL

5mL

1mL

Same as Matrix Gel (standard) with no phenol red added.

Matrix Gel GFR (Growth Factor Reduced)

082701

0827015

082701T

10mL

5mL

1mL

Matrix Gel GFR is suitable for research with organoid culture, in vitro angiogenesis, signal optimization of tubular bone cells, etc. Protein concentration: 8-13mg/mL

Matrix Gel GFR (Growth Factor Reduced), Phenol Red Free

082701

0827015

082701T

10mL

5mL

1mL

Same as Matrix Gel GFR (Growth Factor Reduced)with no phenol red added.

Matrix Gel HC (High Concentration)

082724

0827245

082724T

10mL

5mL

1mL

HC series is more suitable for animal models, such as PDX, CDX, in vivo angiogenesis assays. Protein concentration: 16-26mg/mL.

Matrix Gel HC (High Concentration), Phenol Red Free

082726

0827265

082726T

10mL

5mL

1mL

Same as Matrix Gel HC (High Concentration) with no phenol red added.

Matrix Gel HC GFR

082721

0827215

082721T

10mL

5mL

1mL

The Growth Factor Reduced version of high concentration gel, is specially optimized formular.  It can be used as an alternative of HC gel. HC gel is more suitable for animal models, such as PDX, CDX, in vivo angiogenesis assays. Protein concentration: 16-26mg/mL.

Matrix Gel HC GFR, Phenol Red Free

082723

0827235

082723T

10mL

5mL

1mL

Same as Matrix Gel HC GFR with no phenol red added.

Matrix Gel for iPSC

082777

0827775

08277T

10mL

5mL

1mL

Matrix Gel for iPSC is a mature growth substrate for feeder-free hES culture. It is used in combination with specific media to culturize stem cells (e.g, hESC, iPSC). The gel provides the reproducibility and consistency required for trophoblast culture of human embryonic stem cells and induced pluripotent stem cells, as well as can also be used in vivo differentiation studies, such as teratoma formation Protein concentration: 8-13mg/mL.

Matrix Gel for Organoid Culture

082755

0827555

082755T

10mL

5mL

1mL

This product is suggested to be used for the establishment of organoid models. Such as, mouse intestinal organoids, lung organoids, human brain organoids, tumor organoids, etc. Matrix Gel for Organoid Culture can ensure the effectiveness and reproducibility of experiments. Protein concentration: 8-13mg/mL.

Reference applications and data sets
iPSC Culture
Picture
​Matrix Gel for iPSC (Cat. 082777) 1:100 dilution coating the plates, supports stable passage of EPSCs (extended pluripotent stem cells) from P1 to P4.
​Human Tumor Organoid Culture
Picture
Human tumor organoids (left - colorectal cancer organoids and right - gastric cancer organoids) can grow well in Matrix Gel for Organoid Culture (Cat. 082755).
Picture
Human tumor organoids (left - breast cancer organoids and right - hepatocellular carcinoma organoids) can grow well in Matrix Gel GFR (Growth Factor Reduced), Phenol Red Free (Cat. No. AC-M082703).
Mouse Organoid
Picture
Mouse intestinal organoid, liver ductal organoid and airway organoid can also grow well in Matrix Gel GFR (Growth Factor Reduced), Phenol Red Free (Cat. No. AC-M082703).
Tumor Cell Invasion
Picture
The invasion effect of 5×10^4 SGC-7901 was detected for 24 hours at different concentrations of Matrix Gel (standard) (Cat. 082704) and it was clear that the number of successfully invaded cells decreased significantly as the concentration of Matrix Gel increased.
Picture
The invasion effect of 5×10^4 SGC-7901 was detected for 24 hours at different concentrations of Mogengel (Cat. No. AC-M082706) and it was clear that the number of successfully invaded cells decreased significantly as the concentration of Matrix Gel increased.
Tumorigenesis Assay
Picture
Matrix Gel HC (Cat. 082724) significantly promotes the proliferation of tumor cells under the skin in nude mice.
Angiogenesis Assay
Picture
The complete medium and HUVECs suspension (2×104 /well in 96 wells) can form the excellent tubular structure on 100% and 67% Matrix Gel (standard) (Cat. 082704) at 4h, which can be quickly performed for subsequent staining and angiogenesis analysis.
Picture
The complete medium and HUVECs suspension (2×104 /well in 96 wells) can form the excellent tubular structure on 100% and 67% Matrix Gel, Phenol Red Free (082706) at 4h, which can be quickly performed for subsequent staining and angiogenesis analysis.
In Vivo Angiogenesis
Picture
VEGF and Heparin could promote the in vivo angiogenesis through in vivo assay by Matrix Gel HC GFR, Phenol Red Free. (Cat. 082723).
List of Publications

2024
Wang, D. et al. (2024) 'Muscone abrogates breast cancer progression through tumor angiogenic suppression via VEGF/PI3K/Akt/MAPK signaling pathways,' Cancer Cell International, 24(1). https://doi.org/10.1186/s12935-024-03401-6.
Sheng, N. et al. (2024) 'SOX9 promotes stemness in the CAL27 cell line of tongue squamous cell carcinoma,' Cell Biochemistry and Function, 42(3). https://doi.org/10.1002/cbf.4000.
Ju, Y. et al. (2024) 'Microenvironment remodeling Self-Healing hydrogel for promoting Flap survival,' Biomaterials Research/Biomaterials Research, 28. https://doi.org/10.34133/bmr.0001.
Wang, D. et al. (2024b) 'Muscone abrogates breast cancer progression through tumor angiogenic suppression via VEGF/PI3K/Akt/MAPK signaling pathways,' Cancer Cell International, 24(1). https://doi.org/10.1186/s12935-024-03401-6.
Lin, H. et al. (2024) 'Patient tissue-derived FGFR4-variant and wild-type colorectal cancer organoid development and anticancer drug sensitivity testing,' Heliyon, 10(10), p. e30985. https://doi.org/10.1016/j.heliyon.2024.e30985.
 
2023
Ji, L. et al. (2022) 'Insights from DOCK2 in cell function and pathophysiology,' Frontiers in Molecular Biosciences, 9. https://doi.org/10.3389/fmolb.2022.997659.
YanYun, J. et al. (2023) 'Compatibility of Calycosin-Tanshinone IIA improves Ang II-induced renal artery endothelial cell dysfunction through lncRNA-mRNA coexpression network,' Research Square (Research Square) [Preprint]. https://doi.org/10.21203/rs.3.rs-3205631/v1.
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