Phosphate-Buffered Saline (10x stock, 1L) |
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Alias: PBS, DPBS (when Ca and Mg
free) |
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Reagent |
Amount |
Final Concentration |
NaCl |
80 g |
1.37 M |
KCl |
2.0 g |
27 mM |
Na2HPO4 |
14.4 g |
100 mM |
KH2PO4 |
2.4 g |
18 mM |
If necessary, PBS may be
supplemented with the following: |
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CaCl2•2H2O |
1.33 g |
10 mM |
MgCl2•6H2O |
1.0 g |
5 mM |
Directions Mix
all of the above with 800mL ultra-pure water and dissolve well till no
particle observed. Adjust
pH to 7.2, 7.4 or 7.6 with 5M HCl. Then
add ultra-pure water till 1L. |
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Sterilization Filter
with 0.22um membrane filter. Or
sterilize it by autoclaving for 20 min at 121°C or 15 psi (1.05 kg/cm2) |
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Usage Cell
study, Immunology assay, Molecular biology, Protein analysis, Physiology
test, Histology test |
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Storage Room
temperature, avoid direct sunshine. |