Customized Assay Kit Development Service
50+ Immunoassay projects2 IVD projects are in registration |
At Bio Bench, assay kit development services are backed by talented technical team and years of management experience. Till June, 2019, over 50 immunoassay projects have been developed and/or manufactured then distributed to the clients across the globe. Thanks to our client, we have successfully developed these kit for lab research, IVD (In Vitro Diagnostic), food safety, veterinary drug residue, bioprocess, etc.
With Bio Bench capacity, we are also proud to provide a series of immunoassay kit materials covering IVD, cell signaling, immune check point, cluster of differentiation, interleukin, etc. (Download: Bio Bench reconstructed protein). Bio Bench has scientists that are expert in the development of immunoassays of different formats. Whether you are developing an ELISA/EIA or immunohistochemistry, IHC/ICC assay, western blot or flow cytometry assay, our services and products are high-performance formulations that provide a quicker path to commercialization. With our reliable technology, know-how protocols and state-of-the-art facilities, we are capable to develop the most sensitive, reproducible immunoassays and immunodiagnostic kit.
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How we develop an immunoassay?
Basically, we gather the right components and create a good protocol. An immunoassay kit which have good performance is composed of many influencing factors, including binder, substrate, buffer, protocol, etc. To ensure the results of the assay are robust and accurate, Bio Bench technical team will optimize the assay systematically and test all components and variables. Bio Bench helps you to choose or develop each component and create a functional, specific, sensitive, stable and reproducible protocol. |
How is your project proceeded?
Supported Applications
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ELISA (Enzyme-Linked Immuno Sorbent Assay) method is still the most widely used for the quantitative measurement among many industries. Based on the method of detecting, ELISA assay are classified into sandwich ELISA, competitive ELISA direct ELISA and indirect ELISA. These strategies are also developed based on different type of sample. Sandwich ELISA requires experienced team to discover the functional antibody pair. The methods to develop signal is improved during the past 3 decades, the most commonly applied methods including ELISAs Colorimetric ELISA, Chemiluminescent ELISA, Electrochemiluminescence, etc. |
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Western Blot and Dot Blot The western blot assay is composed of a gel electrophoresis to separate native proteins, followed by an electrophoretic transfer onto a membrane and an immunostaining procedure to visualize a certain protein on the blot membrane. In research field WB is still very well accepted to detect the existence of certain indicator. With conjugation with fluorosphors, the detection ability can even be improved 50 times compare to the conventional film developing method. |
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IHC (Immunohistochemistry), ICC (Immunocytochemistry) and IF (Immunofluorescence) methods are wide used for qualitative and quantitative measurement of tissure samples. These methods exploit the principle of antibodies binding specifically to antigens in situ in either freshly obtained or dried biological samples. The detection of the interaction can be accomplished by using either antibody or naturally binding protein. The signal of the interaction can be developed through either chemical substrate or fluorescent. The results are commonly in image and in data, which allowing researchers to conduct further study based on proven result. |
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Flow Cytometry is a technology that is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. It allows user to detect up to 120 different factors at the same time by exploiting antibodies conjugated with different fluorospheres that exhibit different excitation, emission and/or even the brightness. By using beads as attachment material, there are even more flow cytometry methods are developed accordingly. |
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HCS (High Content Screening assay) is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell in a desired manner. It is normally a image based assay or the signal exhibit multiplexed factors of a single cell. The effective components of the assay including the antibody, fluorospheres, the experiment buffer and the protocol. Each of the component shall be developed according to the specific application and also verified by the real situation. |
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Assay Kit Material Development and Production
In order to guarantee the quality of the assay kit, to develop new antibody and protein shall be taken into consideration. The commercial antibody and protein are commonly designed for specific platform, it either not compatible with your application or takes extra time to find the best matching. Also Bio Bench has established pilot scale production capacity up to kg level protein.
Protein Expression Service
Monoclonal Antibody Development Service
In order to guarantee the quality of the assay kit, to develop new antibody and protein shall be taken into consideration. The commercial antibody and protein are commonly designed for specific platform, it either not compatible with your application or takes extra time to find the best matching. Also Bio Bench has established pilot scale production capacity up to kg level protein.
Protein Expression Service
Monoclonal Antibody Development Service
