Service
and Timeline |
Service
Description |
Deliverables |
Price |
P110111 E.coli-Ready 3 – 4 Weeks |
1. Transformation and clone selection. 2. Small volume e.coli
fermentation and Expression test. 3. Affinity purification (1-step). 4. Protein denaturing and renaturing if the protein is
expressed in the inclusion body. 5. SDS-PAGE test. 6. QC and report. Addition: > Gene codon optimization, synthesis and
reconstructed plasmid. |
1. 0.2mg-1mg purified protein. 2. Data and QC report. |
|
P110112 E.coli-StepUp 7 – 9 Weeks |
1. Condon optimization and gene
synthesis (1000bp free). 2. Transformation and clone selection. 3. Midi volume (xxx mL) fermentation
and expression test: up to 8 different conditions. 4. Protein denaturing and
renaturing if the protein is expressed in the inclusion body. 5. Protein purification (1-step affinity
purification). 6. WB and SDS-PAGE test. 7. QC and report. |
1. 0.5mg-5mg purified protein. 2. Data and QC report. |
|
P110113 E.coli-Comfo 7 – 9 Weeks |
1. Condon optimization and gene synthesis (free of charge). 2. Minimum 2 expression strains and/or vectors will be tested: transformation
and clone selection. 3. 200mL - 1L fermentation and expression condition test: up to
16 different conditions. 4. Protein denaturing and renaturing if the protein is expressed
in the inclusion body. 5. Protein purification with 2 steps, purity polishing to >85%. 6. WB and SDS-PAGE test. 7. QC and report. |
1. 1mg - 20mg purified protein. 2. Data and QC report. |
|
P110102 1L E.coli fermentation 1 Week |
1L E.coli fermentation and protein
purification for 1 clone. |
Up to 500mg purified protein. |
|
P110103 5L E.coli fermentation 1 – 1.5 Week |
5L E.coli fermentation and protein
purification for 1 clone. |
Up to 2500mg purified protein. |
Service type Content |
E.coli Ready |
E.coli StepUp |
E.coli Comfo |
Codon
optimization |
- |
✓ |
✓ |
Gene
synthesis |
- |
✓ |
✓ |
Multiple
vector or multiple strain test |
- |
- |
✓ |
Expression
temperature test: HighT °C |
✓ |
✓ |
✓ |
Expression
temperature test: LowT °C |
- |
✓ |
✓ |
Expression
temperature test: MediumT °C |
- |
- |
✓ |
Expression
duration test (time points) |
1 |
2 |
3 |
Multiple
concentration of inducing reagent |
1 |
2 |
2 |
Fermentation
volume |
80mL |
200mL |
200mL – 1L |
Affinity
purification |
✓ |
✓ |
✓ |
2nd
purification: purity enhancement |
- |
- |
✓ |
SDS-PAGE
analysis |
✓ |
✓ |
✓ |
WB
analysis |
- |
✓ |
✓ |
Data,
QC and report |
✓ |
✓ |
✓ |
Video
meeting support |
- |
- |
✓ |
Purified
protein |
0.5mg – 2mg |
0.5mg – 5mg |
1mg – 20mg |
|
Optional Service 1. Tag remove 2. Endotoxin removal 3. 10L~4000L fermentation 4. Inclusion body protein denaturing and renaturing 5. Western Blot for protein confirmation 6. Mass Spectrometry More information: Please Email to [email protected] if you need special service for E. Coli Expression service. |
List of Vectors |
Commercial vectors, low temperature expression vectors. |
List of Expression Strain |
Special stains for your special protein: High expression strain, low temperature strain, toxic protein expression strain, rare codons expression strain and disulfide bonds expression strain. |