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E. coli expression system is the most widely used expression method. Advantages of E.coli expression system includes: clear genetic background and metabolic pathway, mature method for expression regulation and time-saving cell culture. 

​Service highlight:
1. Up to 95% soluble protein expression.
2. Multiple choices for vectors, E.coli strains, tags and Restriction Enzyme cutting sites. Vector bank for different target protein: pET series, pBAD series and pCold series.

Service and Timeline	Service Description	Deliverables	Price
P110111 E.coli-Ready 3 – 4 Weeks	1. Transformation and high expression monoclone selection. 2. 80mL fermentation. 3. Expression condition test: 2 different conditions. 4. Protein purification (1-step affinity purification). 5. SDS-PAGE test. 6. QC and report.	1. 0.5mg-2mg purified protein. 2. Data and QC report.	€ 999.00 Inquiry​
P110112 E.coli-StepUp 7 – 9 Weeks	1. Condon optimization and gene synthesis (1000bp free). 2. Transformation and high expression monoclone selection. 3. Expression condition test: up to 16 different conditions. 4. 200mL fermentation. 5. Protein purification (1-step affinity purification). 6. WB and SDS-PAGE test. 7. QC and report.	1. 0.5mg-5mg purified protein. 2. Data and QC report.	Inquiry​
P110113 E.coli-Comfo 7 – 9 Weeks	1. Condon optimization and gene synthesis (free of charge). 2. Minimum 2 expression strains or vectors will be tested: transformation and high expression monoclone selection. 3. Expression condition test: up to 36 different conditions. 4. 200mL - 1L fermentation. 5. Protein purification with 2 steps, purity polishing to >90%. 6. WB and SDS-PAGE test. 7. QC and report. 8. One time video meeting.	1. 1mg - 20mg purified protein. 2. Data and QC report.	Inquiry​
P110202 1L fermentation 1 Week	1L fermentation and protein purification for 1 clone.	Up to 500mg purified protein.	Inquiry​
P110203 5L fermentation 1 – 1.5 Week	5L fermentation and protein purification for 1 clone.	Up to 2500mg purified protein.	Inquiry​

 

Optional Service 1. Tag remove 2. Endotoxin removal 3. 10L~4000L fermentation 4. Inclusion body protein denaturing and renaturing 5. Western Blot for protein confirmation 6. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service for E. Coli Expression service.

Comparison of different types of service

Service type Content	E.coli Ready	E.coli StepUp	E.coli Comfo
Codon optimization	-	✓	✓
Gene synthesis	-	✓	✓
Multiple vector or multiple strain test	-	-	✓
Expression temperature test: HighT °C	✓	✓	✓
Expression temperature test: LowT °C	-	✓	✓
Expression temperature test: MediumT °C	-	-	✓
Expression duration test (time points)	1	2	3
Multiple concentration of inducing reagent	1	2	2
Fermentation volume	80mL	200mL	200mL – 1L
Affinity purification	✓	✓	✓
2nd purification: purity enhancement	-	-	✓
SDS-PAGE analysis	✓	✓	✓
WB analysis	-	✓	✓
Data, QC and report	✓	✓	✓
Video meeting support	-	-	✓
Purified protein	0.5mg – 2mg	0.5mg – 5mg	1mg – 20mg
	Inquiry​	Inquiry​	Inquiry​

 

List of Vectors	Commercial vectors, low temperature expression vectors.
List of Expression Strain	Special stains for your special protein: High expression strain, low temperature strain, toxic protein expression strain, rare codons expression strain and disulfide bonds expression strain.