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e.coli protein expression system
bacilus subtilis protein expression system
yeast protein expression system
baculovirus insect cell protein expression system
mammalian HEK293 CHO protein expression system
antibody production

e.coli protein expression system
E. coli expression system is the most widely used expression method. Advantages of E.coli expression system includes: clear genetic background and metabolic pathway, mature method for expression regulation and time-saving cell culture. 

E.coli Expression Standard Service

​Service highlight:
1.   Good solution for challenging proteins and other difficult projects.
2.  Affordable, flexible and creditable service tailored to match your research requirements.
3.  
Fast lead time - start from 5 weeks your protein will be delivered.
4.  
State-of-the-art technologies - codon optimization and protein refolding.
5.  
One-stop service platform from gene to protein.
​

2. Multiple choices for vectors, E.coli strains, tags and Restriction Enzyme cutting sites. Vector bank for different target protein: pET series, pBAD series and pCold series.
3. Multiple tag options: His, GST, Trx, SUMO, Flag, HA, as well as tandem tags.

Service and Timeline

Service Description

Deliverables

Price

P110111

E.coli-Ready

3 – 4 Weeks

1. Transformation and high expression monoclone selection.

2. 80mL fermentation.

3. Expression condition test: 2 different conditions.

4. Protein purification (1-step affinity purification).

5. SDS-PAGE test.

6. QC and report.

1. 0.1mg-1mg purified protein.

2. Data and QC report.

Inquiry

Inquiry​

P110112

E.coli-StepUp

7 – 9 Weeks

1. Condon optimization and gene synthesis (1000bp free).

2. Transformation and high expression monoclone selection.

3. Expression condition test: up to 16 different conditions.

4. 200mL fermentation.

5. Protein purification (1-step affinity purification).

6. WB and SDS-PAGE test.

7. QC and report.

1. 0.5mg-5mg purified protein.

2. Data and QC report.

Inquiry​

P110113

E.coli-Comfo

7 – 9 Weeks

1. Condon optimization and gene synthesis (free of charge).

2. Minimum 2 expression strains or vectors will be tested: transformation and high expression monoclone selection.

3. Expression condition test: up to 36 different conditions.

4. 200mL - 1L fermentation.

5. Protein purification with 2 steps, purity polishing to >90%.

6. WB and SDS-PAGE test.

7. QC and report.

8. One time video meeting.

1. 1mg - 20mg purified protein.

2. Data and QC report.

Inquiry​

P110202

1L fermentation

1 Week

1L fermentation and protein purification for 1 clone.

Up to 500mg purified protein.

Inquiry​

P110203

5L fermentation

1 – 1.5 Week

5L fermentation and protein purification for 1 clone.

Up to 2500mg purified protein.

Inquiry​

 

Comparison of different types of service

                                Service type

Content

E.coli

Ready

E.coli

StepUp

E.coli

Comfo

Codon optimization

-

✓

✓

Gene synthesis

-

✓

✓

Multiple vector or multiple strain test

-

-

✓

Expression temperature test: HighT °C

✓

✓

✓

Expression temperature test: LowT °C

-

✓

✓

Expression temperature test: MediumT °C

-

-

✓

Expression duration test (time points)

1

2

3

Multiple concentration of inducing reagent

1

2

2

Fermentation volume

80mL

200mL

200mL – 1L

Affinity purification

✓

✓

✓

2nd purification: purity enhancement

-

-

✓

SDS-PAGE analysis

✓

✓

✓

WB analysis

-

✓

✓

Data, QC and report

✓

✓

✓

Video meeting support

-

-

✓

Purified protein

0.5mg – 2mg

0.5mg – 5mg

1mg – 20mg

 

Inquiry​

Inquiry​

Inquiry​

 

Optional Service
1. Tag remove
2. Endotoxin removal
3. 10L~4000L fermentation
4. Inclusion body protein denaturing and renaturing
5. Western Blot for protein confirmation
6. Mass Spectrometry
More information: Please Email to info@bio-bench.com if you need special service for E. Coli Expression service.
List of Vectors
Commercial vectors, low temperature expression vectors.
List of Expression Strain
Special stains for your special protein:
High expression strain, low temperature strain, toxic protein expression strain, rare codons expression strain and disulfide bonds expression strain.
bio bench your reliable cro partner aaalac gmp

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  • Antibody
    • Polyclonal Ab
    • Hybridoma mAb
    • Phage Display mAb
    • Human Antibody
    • Antibody Engineering
  • Protein
    • E.coli
    • B.Subtilis
    • Yeast
    • Insect Cell
    • Mammalian
    • Antibody Production
    • Stable Cell Line
  • Drug Discovery
  • Assay Kit Service
  • Catalog Product
    • AccuPAGE™ Bis-Tris Precast PAGE Gel
    • AccuPAGE™ Tris-Glycine Precast PAGE Gel
    • COVID-19
    • Covid-19 Kit
  • Support
    • Buffer and Reagent
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