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e.coli protein expression system
bacilus subtilis protein expression system
yeast protein expression system
baculovirus insect cell protein expression system
mammalian HEK293 CHO protein expression system
antibody production

e.coli protein expression system
E. coli expression system is the most widely used expression method. Advantages of E.coli expression system includes: clear genetic background and metabolic pathway, mature method for expression regulation and time-saving cell culture. 

​Service highlight:
1. Up to 95% soluble protein expression.
2. Multiple choices for vectors, E.coli strains, tags and Restriction Enzyme cutting sites. Vector bank for different target protein: pET series, pBAD series and pCold series.
Service and
Time Line
Service Description
Deliverables
Price
P110100
Standard
​23~56 days
1. Condon optimization and gene synthesis.
2. Subcloning and transformation.
3. Expression condition optimization.
4. High expression level monoclone selection.
5. 80mL fermentation and protein purification.
•1 clone.
•1 reconstructed plasmid.
•1mg~10mg purified protein.
•Data and QC report.
€1299
Inquiry​
P110201
Extra 1 clone
1 day

Extra 1 clone will be selected.
•1 clone.
•1mg~10mg purified protein.
•Data and QC report.
€399
Inquiry​
P110202
1L fermentation
3~5 days

1L fermentation and protein purification for 1 clone.
•5mg-500mg protein.
€899
Inquiry​
P110203
5L fermentation
5~7 days
5L fermentation and protein purification for 1 clone.
•50mg-2500mg protein.
Inquiry​
Optional Service
1. Tag remove
2. 10L~4000L fermentation
3. Inclusion body protein denaturing and renaturing
4. Western Blot for protein confirmation
5. Mass Spectrometry
More information: Please Email to info@bio-bench.com if you need special service for E. Coli Expression service.
List of Vectors
Commercial vectors, low temperature expression vectors.
List of Expression Strain
Special stains for your special protein:
High expression strain, low temperature strain, toxic protein expression strain, rare codons expression strain and disulfide bonds expression strain.

bio bench your reliable cro partner aaalac gmp

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Hybridoma mAb
Phage Display mAb
Human Naive antibody Library
mAb Engineering
​

Protein

E. coli
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Yeast
Insect Cell
Mammalian
​Antibody Production
​Protein Production
​
Protein Engineering

Drug Discovery

IVD Kit Development

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  • Antibody
    • Hybridoma mAb
    • Phage Display mAb
    • Human Naive Antibody Library
    • Antibody Engineering
  • Protein
    • E.coli
    • B.Subtilis
    • Yeast
    • Insect Cell
    • Mammalian
    • Antibody Production
  • Drug Discovery
  • Assay Kit Service
  • Catalog Product
    • COVID-19
    • Covid-19 Kit
  • Support
    • Buffer and Reagent
    • Knowledge base
  • Contact Us
    • Company
    • Career
    • Event