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E. coli expression system is the most widely used expression method. Advantages of E.coli expression system includes: clear genetic background and metabolic pathway, mature method for expression regulation and time-saving cell culture.
Service highlight: 1. Up to 95% soluble protein expression. 2. Multiple choices for vectors, E.coli strains, tags and Restriction Enzyme cutting sites. Vector bank for different target protein: pET series, pBAD series and pCold series. |
Service and Time Line |
Service Description |
Deliverables |
Price |
P110100 Standard 23~56 days |
1. Condon optimization and gene synthesis. 2. Subcloning and transformation. 3. Expression condition optimization. 4. High expression level monoclone selection. 5. 80mL fermentation and protein purification. |
•1 clone. •1 reconstructed plasmid. •1mg~10mg purified protein. •Data and QC report. |
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P110201 Extra 1 clone 1 day |
Extra 1 clone will be selected. |
•1 clone. •1mg~10mg purified protein. •Data and QC report. |
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P110202 1L fermentation 3~5 days |
1L fermentation and protein purification for 1 clone. |
•5mg-500mg protein. |
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P110203 5L fermentation 5~7 days |
5L fermentation and protein purification for 1 clone. |
•50mg-2500mg protein. |
Optional Service 1. Tag remove 2. 10L~4000L fermentation 3. Inclusion body protein denaturing and renaturing 4. Western Blot for protein confirmation 5. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service for E. Coli Expression service. |
List of Vectors |
Commercial vectors, low temperature expression vectors. |
List of Expression Strain |
Special stains for your special protein: High expression strain, low temperature strain, toxic protein expression strain, rare codons expression strain and disulfide bonds expression strain. |
