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Baculovirus expression system is widely used in protein production. Compare with mammalian expression system, baculovirus-insect Expression System has some unique advantages: higher expression level, simple clone selection and high level post translational modification.

Service highlight:
1. Team members have average 10 years’ experience in Baculovirus-insect expression system, 99% success rate.
2. Multiple expression cell lines and vectors.
3. Up to 200L fermentation volume, possible reach 100g protein production.

Service and Timeline	Service Description	Deliverables	Price
P110411 Insect-Ready 5 - 7 Weeks	1. P1 generation virus production. 2. 80mL cell culture. 3. Expression condition test: up to 3 different conditions. 4. Protein purification (1-step affinity purification). 5. SDS-PAGE test. 6. QC and report.	1. 0.3mg-1mg purified protein. 2. Data and QC report.	Inquiry​
P110412 Insect-StepUp 9 – 11 Weeks	1. Condon optimization and gene synthesis (1000bp free). 2. P1 generation virus production (low titer). 3. P2 generation virus production (high titer). 4. 200mL cell culture. 5. Expression condition test: up to 6 different conditions. 6. Protein purification (1-step affinity purification). 7. WB and SDS-PAGE test. 8. QC and report.	1. 0.5mg-5mg purified protein. 2. Data and QC report.	Inquiry​
P110413 Insect-Comfo 9 – 11 Weeks	1. Condon optimization and gene synthesis (free of charge). 2. 2 expression vectors will be tested. 3. P1 generation virus production (low titer). 4. P2 generation virus production (high titer). 5. 200mL - 1L cell culture. 6. Protein purification with 2 steps, purity polishing to >90%. 7. WB and SDS-PAGE test. 8. QC and report. 9. One time video meeting.	1. 1mg - 10mg purified protein. 2. Data and QC report.	Inquiry​
P110402 1L Cell Culture 2 Weeks	1L fermentation and protein purification for 1 clone.	Up to 30mg purified protein.	Inquiry​

 

Optional Service 1. Tag remove 2. 5L~200L fermentation 3. Western Blot for protein confirmation 4. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service for Baculovirus-insect Expression System.

Comparison of different types of service

Service type Content	Insect cell Ready	Insect cell StepUp	Insect cell Comfo
Codon optimization	-	✓	✓
Gene synthesis	-	✓	✓
Multiple vector or multiple cell line test	-	-	✓
Expression temperature test: HighT °C	✓	✓	✓
P1 generation virus	✓	✓	✓
P2 generation virus	-	✓	✓
Expression duration test (time points)	1	2	2
Fermentation volume	80mL	200mL	200mL – 1L
Affinity purification	✓	✓	✓
2nd purification: purity enhancement	-	-	✓
SDS-PAGE analysis	✓	✓	✓
WB analysis	-	✓	✓
Data, QC and report	✓	✓	✓
Video meeting support	-	-	✓
Purified protein	0.3mg – 2mg	0.5mg – 5mg	1mg – 10mg
	Inquiry​	Inquiry​	Inquiry​

 

List of Vectors	Commercial vectors and Bio Bench improved vectors. Please inquire for the up-to-date list.
List of Expression Strain	Sf9, Sf21, Hi5. Please inquire for the up-to-date list.