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e.coli protein expression system
bacilus subtilis protein expression system
yeast protein expression system
baculovirus insect cell protein expression system
mammalian HEK293 CHO protein expression system
antibody production

bacillus subtilis protein expression
Compare with E.coli expression system, Bacillus Subtilis has obvious advantages: low codon bias, high possibility to express proteins with high solubility and high bioactivity, no endotoxin in the purified protein.

​Service highlight:
1. Bio Bench provides vectors for either intracellular expression or secretory expression.
2. Improved transformation method guarantee a success rate of minimum 90%.
3. Up to 4000L fermentation system and downstream protein purification.
Service and
Time Line
Service Description
Deliverables
Price
P110200
Standard
31~45 days

1. Condon optimization and gene synthesis.
2. Subcloning and transformation.
3. Expression condition optimization.
4. High expression level monoclone selection.
5. 200mL fermentation and protein purification.

•1 clone.
•1 reconstructed plasmid.
•1mg-10mg purified protein.
•Data and QC report.
€1999
Inquiry​
P110201
Extra 1 clone
1 day

Extra 1 clone will be selected.
•1 clone.
•1mg-10mg purified protein.
•Data and QC report.
€399
Inquiry​
P110202
1L fermentation
3~5 days

1L fermentation and protein purification for 1 clone.
•5mg-500mg protein.
€899
Inquiry​
P110203
5L fermentation
5~7 days

5L fermentation and protein purification for 1 clone.
•50mg-2500mg protein.
Inquiry​
Optional Service
1. Tag remove
2. 10L~4000L fermentation
3. Inclusion body protein denaturing and renaturing
4. Western Blot for protein confirmation
5. Mass Spectrometry
More information: Please Email to info@bio-bench.com if you need special service for Bacillus Subtilis Expression System.
List of Vectors
Bio Bench qualified vectors for both secretory expression and intracellular expression.
List of Expression Strain
Bio Bench qualified strains for both secretory expression and intracellular expression.
bio bench your reliable cro partner aaalac gmp

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Hybridoma mAb
Phage Display mAb
Human Naive antibody Library
mAb Engineering
​

Protein

E. coli
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Yeast
Insect Cell
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​Antibody Production
​Protein Production
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Protein Engineering

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  • Antibody
    • Hybridoma mAb
    • Phage Display mAb
    • Human Naive Antibody Library
    • Antibody Engineering
  • Protein
    • E.coli
    • B.Subtilis
    • Yeast
    • Insect Cell
    • Mammalian
    • Antibody Production
  • Drug Discovery
  • Assay Kit Service
  • Catalog Product
    • COVID-19
    • Covid-19 Kit
  • Support
    • Buffer and Reagent
    • Knowledge base
  • Contact Us
    • Company
    • Career
    • Event