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Compare with E.coli expression system, Bacillus Subtilis has obvious advantages: low codon bias, high possibility to express proteins with high solubility and high bioactivity, no endotoxin in the purified protein.

​Service highlight:
1. Bio Bench provides vectors for either intracellular expression or secretory expression.
2. Improved transformation method guarantee a success rate of minimum 90%.
3. Up to 4000L fermentation system and downstream protein purification.

Service and Timeline	Service Description	Deliverables	Price
P110211 B.subtilis-Ready 3 – 4 Weeks	1. Transformation and clone selection. 2. Small volume B.subtilis culture and expression test. 3. Affinity purification (1-step). 4. SDS-PAGE test. 5. QC and report. Addition: > Gene codon optimization, synthesis and reconstructed plasmid.	1. 0.2mg-1mg purified protein. 2. Data and QC report.	Inquiry​
P110212 B.subtilis-StepUp 7 – 9 Weeks	1. Condon optimization and gene synthesis (1000bp free). 2. Transformation and clone selection. 3. Midi volume B.subtilis culture and expression test: up to 12 different conditions. 4. Affinity purification (1-step). 5. WB and SDS-PAGE test. 6. QC and report.	1. 0.5mg-5mg purified protein. 2. Data and QC report.	Inquiry​
P110213 B.subtilis-Comfo 7 – 9 Weeks	1. Condon optimization and gene synthesis (free of charge). 2. Minimum 2 expression strains and/or vectors will be tested: transformation and clone selection. 3. 200mL - 1L B.subtilis and expression condition test: up to 24 different conditions. 4. Protein purification with 2 steps, purity polishing to >85%. 5. WB and SDS-PAGE test. 6. QC and report. 7. One time video meeting.	1. 1mg - 20mg purified protein. 2. Data and QC report.	Inquiry​
P110202 1L B.subtilis fermentation 3~5 days	1L B.subtilis fermentation and protein purification for 1 clone.	Up to 500mg purified protein.	Inquiry​
P110203 5L fermentation 5~7 days	5L B.subtilis fermentation and protein purification for 1 clone.	Up to 2500mg purified protein.	Inquiry​

 

Optional Service 1. Tag remove ​2. Endotoxin removal 3. 10L~4000L fermentation 4. Inclusion body protein denaturing and renaturing 5. Western Blot for protein confirmation 6. Mass Spectrometry More information: Please Email to [email protected] if you need special service for Bacillus Subtilis Expression System.

Comparison of different types of service

Service type Content	B.subtilis Ready	B.subtilis StepUp	B.subtilis Comfo
Codon optimization	-	✓	✓
Gene synthesis	-	✓	✓
Multiple vector or multiple strain test	-	-	✓
Expression temperature test: HighT °C	✓	✓	✓
Expression temperature test: LowT °C	-	✓	✓
Expression temperature test: MediumT °C	-	-	✓
Expression duration test (time points)	1	2	3
Multiple concentration of inducing reagent	1	2	2
Fermentation volume	80mL	200mL	200mL – 1L
Affinity purification	✓	✓	✓
2nd purification: purity enhancement	-	-	✓
SDS-PAGE analysis	✓	✓	✓
WB analysis	-	✓	✓
Data, QC and report	✓	✓	✓
Video meeting support	-	-	✓
Purified protein	0.3mg – 2mg	0.5mg – 5mg	1mg – 20mg
	Inquiry​	Inquiry​	Inquiry​

 

List of Vectors	Bio Bench qualified vectors for both secretory expression and intracellular expression.
List of Expression Strain	Bio Bench qualified strains for both secretory expression and intracellular expression.