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Compare with E.coli expression system, Bacillus Subtilis has obvious advantages: low codon bias, high possibility to express proteins with high solubility and high bioactivity, no endotoxin in the purified protein.
Service highlight: 1. Bio Bench provides vectors for either intracellular expression or secretory expression. 2. Improved transformation method guarantee a success rate of minimum 90%. 3. Up to 4000L fermentation system and downstream protein purification. |
Service and Time Line |
Service Description |
Deliverables |
Price |
P110200 Standard 31~45 days |
1. Condon optimization and gene synthesis. 2. Subcloning and transformation. 3. Expression condition optimization. 4. High expression level monoclone selection. 5. 200mL fermentation and protein purification. |
•1 clone. •1 reconstructed plasmid. •1mg-10mg purified protein. •Data and QC report. |
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P110201 Extra 1 clone 1 day |
Extra 1 clone will be selected. |
•1 clone. •1mg-10mg purified protein. •Data and QC report. |
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P110202 1L fermentation 3~5 days |
1L fermentation and protein purification for 1 clone. |
•5mg-500mg protein. |
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P110203 5L fermentation 5~7 days |
5L fermentation and protein purification for 1 clone. |
•50mg-2500mg protein. |
Optional Service 1. Tag remove 2. 10L~4000L fermentation 3. Inclusion body protein denaturing and renaturing 4. Western Blot for protein confirmation 5. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service for Bacillus Subtilis Expression System. |
List of Vectors |
Bio Bench qualified vectors for both secretory expression and intracellular expression. |
List of Expression Strain |
Bio Bench qualified strains for both secretory expression and intracellular expression. |
