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Since hybridoma technology was established, the applications of mouse antibody have been very well developed in the research and in the biopharmaceutical industry (drug antibody discovery). The advantages of mouse hybridoma monoclonal antibody include: high affinity, high specificity, mature antibody discovery strategy and easy post modification. Compare to phage display, hybridoma technology is cost-effective for harvesting 1 – 20 clones. It is thus widely used in early stage research and/or in some projects that does not require further antibody engineering.

The application guaranteed monoclonal antibody service is a one-stop-shop service that follows a high standard from the initiation till the delivery result. The bioinformatic team will analyze and design the immunogen according to the protein sequence. Vivo-Prot™ protein expression will be used to generate original conformation protein for immunization and screening. The host animal will then be immunized by a hybrid immunization method involving DNA, peptide, protein and/or cell line together with patent adjuvant. In the end, the serum/supernatant will be tested/validated according to the designated application to guarantee the project success. Bio Bench applies cutting edge technology and know-how SOP to offer the customers reliable support.

Typical process for your antibody project

Phase
Service Options
Deliverables
Timeline
Phase 1
Antigen
  1. Peptide synthesis.
  2. Peptide conjugation.
  3. Gene cloning (if needed).
  4. E.coli expression.
  5. Mammalian cell expression.​
  1. QC Report.
  2. 100μg peptide or 1mg protein.
  3. Cell line (if customer requires).
Peptide: 1 week.
Protein: 7 – 12 weeks.
Phase 2
Immunization
  1. DNA/Peptide/protein/whole cell immunization.
  2. Hybrid immunization.
  3. 3, 5, 7 or 10 BALB/c mouse will be immunized.
  4. Bio Bench enhanced adjuvant.
  5. Extended immunization.
  1. Test bleed report, titer 1:64 000.
  2. 10μL serum sample at 3rd boost (if customer requires).
4 – 8 weeks.
Phase 3
Cell fusion and screening
  1. Indirect ELISA screening.
  2. WB validation.
  3. Capture ELISA.
  4. Competitive ELISA.
  5. FACS screening.
  6. Cell-based ELISA or FACS.
  7. Counter screening.
  8. HTS/HCS screening.
  1. Parental supernatant test report.
  2. 2 mL supernatant (if customer requires).
2 – 3 weeks.
Phase 4
Subcloning, screening and lab scale production
  1. Subclone screening.
  2. Cryopreservation.
  3. Cell banking.
  4. Lab scale production and purification.
  1. Test report.
  2. 5mL supernatant of the subclones.
  3. 2 vials for each clone.
  4. 1mg/clone purified antibody.
2 – 7 weeks.
Phase 5
Guaranteed applications
  1. Sandwich ELISA.
  2. Western blot.
  3. Flow cytometry.
  4. Immunoflurocent asssay.
  1. Test report.
  2. At least 1 clone or 1 pair of clones.
2 – 8 weeks.

Standard – Monocolonal Antibody Service

Service and
Time Line
Guarantee
Starting Material
Deliverables
Price
AB220100
Standard
109 days
1 clone.
ELISA positive guaranteed.
Peptide/protein (Customer provided).
•100μg purified antibody.
•1 hybridoma.
•QC and Report.
Inquiry​
Package detail:
  1. Immunize 3 mice with conjugated antigen.
  2. Serum titer tested by indirect ELISA.
  3. 1 mouse with the best titer is selected for cell fusion.
  4. Primary screening by ELISA : 8 - 12 x 96-well plates.
  5. 2 parental clones will be subcloned (2-3 rounds of limited dilutions) and tested by ELISA.
  6. 1 best clone will be selected to produce 100μg purified antibody.
Email to info@bio-bench.com if you have any question.
ELISA: Enzyme-Linked Immunosorbent Assay, WB: Western Blot, FCS: Flow Cytometry, IF: ImmunoFluorescent assay.
Service and
Time Line
Guarantee
Starting Material
Deliverables
Price
AB220101
Standard
(E.coli expressed antigen)
159 days
1 clone.
E.coli strain.
ELISA guaranteed.
Protein/DNA sequence provided by customer.
E.coli strain will be built by Bio Bench.
•1mg purified antibody.
•500μg purified protein.
•1 hybridoma, within 3 hybridomas free of charge.
•E.coli strain.
•QC and Report.
Inquiry​
Package detail:
  1. Antigen design. Bio Bench will design the most appropriate fragment to be produced.
  2. Gene optimization, synthesis and subcloning.
  3. Build up E.coli strain.
  4. Immunize 3 mice with the purified protein.
  5. Serum titer tested by indirect ELISA.
  6. 1 mouse with the best titer is selected for cell fusion.
  7. Primary sceening by ELISA : 8 - 12 x 96-well plates.
  8. 2 parental clones will be subcloned (2-3 rounds of limited dilutions) and tested by ELISA.
  9. 1 - 3 best clone will be selected to produce 100μg purified antibody.
​Email to info@bio-bench.com if you have any question.
ELISA: Enzyme-Linked Immunosorbent Assay, WB: Western Blot, FCS: Flow Cytometry, IF: ImmunoFluorescent assay.

WB Guaranteed – Monocolonal Antibody Service

Service and
Time Line
Guarantee
Starting Material
Deliverables
Price
AB220103
WB Guaranteed
149 days
1 clone.
WB guaranteed.
Peptide/protein/DNA sequence provided by customer.
E.Coli strain or mammanlian cell line will be built by Bio Bench.
•1mg purified antibody for each clone.
•1 hybridoma, within 3 hybridomas free of charge.
•500μg purified antigen.
•Strain or cell line.
•QC and Report.
Inquiry​
Package detail:
  1. Antigen design. Bio Bench will design the most appropriate fragment to be produced.
  2. Gene optimization, synthesis and subcloning.
  3. Build up mammalian cell line or E.coli strain and the antigen will be purified.
  4. Immunize 5 mice with the purified protein.
  5. Serum titer tested by indirect ELISA and WB.
  6. 2 mice with the best titer are selected for cell fusion.
  7. Primary screening by ELISA : 16 -24 x 96-well plates and positive clones will be validated by WB.
  8. 2 parental clones will be subcloned (2-3 rounds of limited dilutions), all of the clones will be tested by ELISA and validated by WB.
  9. 1 - 3 best clone will be selected to produce 1mg purified antibody.
​Email to info@bio-bench.com if you have any question.
ELISA: Enzyme-Linked Immunosorbent Assay, WB: Western Blot, FCS: Flow Cytometry, IF: ImmunoFluorescent assay.

Sandwich ELISA Guaranteed – Monocolonal Antibody Service

Service and
Time Line
Guarantee
Starting Material
Deliverables
Price
AB220104
Sandwich ELISA Guaranteed
179 days
2 paring clones.
Sandwich ELISA guaranteed.
Protein/DNA sequence provided by customer.
E.Coli strain or mammanlian cell line will be built by Bio Bench.
•1mg purified antibody for each clone.
•2 hybridomas.
•500μg reconstructed protein.
•E.Coli strain or mammanlian cell line.
•QC and Report.
Inquiry​
Package detail:
  1. Antigen design. Bio Bench will design the most appropriate fragment to be produced.
  2. Gene optimization, synthesis and subcloning.
  3. Build up mammalian cell line or E.coli strain and the antigen will be purified.
  4. Immunize 5 mice with proper method (to be determined by Bio Bench).
  5. Serum titer tested by indirect ELISA.
  6. 2 mice with the best titer are selected for cell fusion.
  7. Primary screening by ELISA : 16 -24 x 96-well plates.
  8. 30 parental clones will be subcloned (2-3 rounds of limited dilutions), all of the clones will be tested by ELISA.
  9. 15 clones will be selected and 1mg antibody will be purified.
  10. Test all of these clones by sandwich ELISA and 2 paring clones will be selected.
  11. 1mg antibody will be purified for each clone.
​Email to info@bio-bench.com if you have any question.
ELISA: Enzyme-Linked Immunosorbent Assay, WB: Western Blot, FCS: Flow Cytometry, IF: ImmunoFluorescent assay.

Flow Cytometry Guaranteed – Monocolonal Antibody Service

Service and
Time Line
Guarantee
Starting Material
Deliverables
Price
AB220105
FCS Guaranteed
179 days
1 clone.
FCS guaranteed.
Protein/DNA sequence provided by customer.
Mammanlian cell line will be built by Bio Bench.
•1mg purified antibody for each clone.
•1 hybridoma, within 3 hybridoma free of charge.
•500μg reconstructed protein.
•Mammalian cell line.
•QC and Report.
Inquiry​
Package detail:
  1. Antigen design. Bio Bench will design the most appropriate fragment to be produced.
  2. Gene optimization, synthesis and subcloning. GFP/RFP tagged protein will also be expressed in parallel as experiment control.
  3. Build up mammalian cell line and the antigen will be purified.
  4. Immunize 5 mice with the proper method (to be determined by Bio Bench).
  5. Serum titer tested by FCS.
  6. 2 mice with the best titer are selected for cell fusion.
  7. Primary screening by FCS: 16 -24 x 96-well plates.
  8. 10 parental clones will be subcloned (2-3 rounds of limited dilutions), all of the clones will be tested by FCS.
  9. 1 – 3 clones will be selected and 1mg antibody will be purified for each clone.
​Email to info@bio-bench.com if you have any question.
ELISA: Enzyme-Linked Immunosorbent Assay, WB: Western Blot, FCS: Flow Cytometry, IF: ImmunoFluorescent assay.

IF Guaranteed – Monocolonal Antibody Service

Service and
Time Line
Guarantee
Starting Material
Deliverables
Price
AB220106
IF Guaranteed
179 days
1 clone.
IF guaranteed.
Protein/DNA sequence provided by customer.
Mammanlian cell line will be built by Bio Bench.
•1mg purified antibody for each clone.
•1 hybridoma, within 3 hybridoma free of charge.
•500μg reconstructed protein.
•Mammalian cell line.
•QC and Report.
Inquiry​
Package detail:
  1. Antigen design. Bio Bench will design the most appropriate fragment to be produced.
  2. Gene optimization, synthesis and subcloning. GFP/RFP tagged protein will also be expressed in parallel as experiment control.
  3. Build up mammalian cell line and the antigen will be purified.
  4. Immunize 5 mice with proper method (to be determined by Bio Bench).
  5. Serum titer tested by ELISA and IF.
  6. 2 mice with the best titer are selected for cell fusion.
  7. Primary screening by ELISA or by FCS: 16 -24 x 96-well plates and validated by IF.
  8. 2 parental clones will be subcloned (2-3 rounds of limited dilutions), all of the clones will be tested by ELISA or FCS, and validated by IF.
  9. 1 - 3 clones will be selected and 1mg antibody will be purified.
​​Email to info@bio-bench.com if you have any question.
ELISA: Enzyme-Linked Immunosorbent Assay, WB: Western Blot, FCS: Flow Cytometry, IF: ImmunoFluorescent assay.
Not satisfied with these service? You have a special application? Your material is not common but it is really important?
Please open your email box and send an email to info@bio-bench.com, we will evaluate your project and provide a regarding solution.
We can do more and better!

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