---

Yeast Expression System has the advantages of both prokaryotic system and eukaryotic system: simple gene operation, high quantity of yield and low project cost, fast cell culture, high level post-translational modification and folding. Bio bench provides Saccharomyces cerevisiae and/or Pichia pastoris as host yeast together with a series of vectors.

​Service highlight:
1. High throughput platform for transformed yeast clone selection.
2. Multiple yeast expression vectors and strains meet different requirement. 
3. Up to 200L fermentation system and downstream purification.

Service and Timeline	Service Description	Deliverables	Price
P110311 Yeast-Ready 4 – 6 Weeks	1. Transformation and clone selection. 2. Small volume yeast cell culture and expression test. 3. Affinity purification (1-step) 4. SDS-PAGE test. 5. QC and report. Addition: > Gene codon optimization, synthesis and reconstructed plasmid.	1. 0.2mg-1mg purified protein. 2. Data and QC report.	Inquiry​
P110312 Yeast-StepUp 7 – 9 Weeks	1. Condon optimization and gene synthesis (1000bp free). 2. Transformation and clone selection. 3. Midi volume (XXX mL) yeast cell culture and expression test: up to 6 different conditions. 4. Affinity purification (1-step) 5. WB and SDS-PAGE test. 6. QC and report.	1. 0.5mg-5mg purified protein. 2. Data and QC report.	Inquiry​
P110313 Yeast-Comfo 7 – 9 Weeks	1. Condon optimization and gene synthesis (free of charge). 2. Minimum 2 expression strains and/or vectors will be tested: transformation and clone selection. 3. 200mL - 1L yeast cell culture and expression condition test: up to 18 different conditions. 4. Protein purification with 2 steps, purity polishing to >85%. 5. WB and SDS-PAGE test. 6. QC and report.	1. 1mg - 20mg purified protein. 2. Data and QC report.	Inquiry​
P110302 1L yeast fermentation 3~5 days	1L yeast fermentation and protein purification for 1 clone.	Up to 500mg purified protein.	Inquiry​
P110303 5L yeast fermentation 5~7 days	5L yeast fermentation and protein purification for 1 clone.	Up to 2500mg purified protein.	Inquiry​

 

Optional Service 1. Tag remove 2. 10L~4000L fermentation 3. Western Blot for protein confirmation 4. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service Yeast Expression System.

Comparison of different types of service

Service type Content	Yeast Ready	Yeast StepUp	Yeast Comfo
Codon optimization	-	✓	✓
Gene synthesis	-	✓	✓
Multiple vector or multiple strain test	-	-	✓
Expression temperature test: HighT °C	✓	✓	✓
Expression temperature test: LowT °C	-	-	✓
Expression duration test (time points)	1	3	5 - 7
Multiple concentration of inducing reagent	1	2	2
Fermentation volume	80mL	200mL	200mL – 1L
Affinity purification	✓	✓	✓
2nd purification: purity enhancement	-	-	✓
SDS-PAGE analysis	✓	✓	✓
WB analysis	-	✓	✓
Data, QC and report	✓	✓	✓
Video meeting support	-	-	✓
Purified protein	0.3mg – 2mg	0.5mg – 5mg	1mg – 10mg
	Inquiry​	Inquiry​	Inquiry​

 

List of Vectors	pPIC9k, pPIC3.5, pPICZαA, pAO815, pYES2/NTA. Please inquire for the up-to-date list.
List of Expression Strain	GS115, KM71, X33, INVSC1. Please inquire for the up-to-date list.