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Yeast Expression System has the advantages of both prokaryotic system and eukaryotic system: simple gene operation, high quantity of yield and low project cost, fast cell culture, high level post-translational modification and folding. Bio bench provides Saccharomyces cerevisiae and/or Pichia pastoris as host yeast together with a series of vectors.
Service highlight: 1. High throughput platform for transformed yeast clone selection. 2. Multiple yeast expression vectors and strains meet different requirement. 3. Up to 200L fermentation system and downstream purification. |
Service and Time Line |
Service Description |
Deliverables |
Price |
P110300 Standard 8~12 weeks |
1. Condon optimization gene synthesis. 2. Subcloning and transformation. 3. High expression monoclone selection. 2 clones will be selected for expression test. 4. 200mL fermentation and protein purification. |
•1 clone. •1 reconstructed plasmid. •1mg-10mg purified protein. •Data and QC report. |
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P110301 Extra 1 clone 1 day |
Extra 1 clone will be selected. |
•1 clone. •1mg-10mg purified protein. •Data and QC report. |
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P110302 Antibiotic pressure selection 2 weeks |
A increasing series of antibiotic concentration will be applied to select the stable and resistance clone. |
Value |
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P110303 1L fermentation 2~3 weeks |
1L fermentation and protein purification for 1 clone. |
•50mg-500mg protein. |
Optional Service 1. Tag remove 2. 10L~4000L fermentation 3. Western Blot for protein confirmation 4. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service Yeast Expression System. |
List of Vectors |
pPIC9k, pPIC3.5, pPICZαA, pAO815, pYES2/NTA. Please inquire for the up-to-date list. |
List of Expression Strain |
GS115, KM71, X33, INVSC1. Please inquire for the up-to-date list. |
