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e.coli protein expression system
bacilus subtilis protein expression system
yeast protein expression system
baculovirus insect cell protein expression system
mammalian HEK293 CHO protein expression system
antibody production

yeast protein expression
Yeast Expression System has the advantages of both prokaryotic system and eukaryotic system: simple gene operation, high quantity of yield and low project cost, fast cell culture, high level post-translational modification and folding. Bio bench provides Saccharomyces cerevisiae and/or Pichia pastoris as host yeast together with a series of vectors.

​Service highlight:
1. High throughput platform for transformed yeast clone selection.
2. Multiple yeast expression vectors and strains meet different requirement. 
3. Up to 200L fermentation system and downstream purification.

Service and Timeline

Service Description

Deliverables

Price

P110311

Yeast-Ready

3 – 4 Weeks

1. Transformation and high expression monoclone selection.

2. 80mL fermentation.

3. Expression condition test: up to 3 different conditions.

4. Protein purification (1-step affinity purification).

5. SDS-PAGE test.

6. QC and report.

1. 0.5mg-2mg purified protein.

2. Data and QC report.

Inquiry​

P110312

Yeast-StepUp

7 – 9 Weeks

1. Condon optimization and gene synthesis (1000bp free).

2. Transformation and high expression monoclone selection.

3. Expression condition test: up to 18 different conditions.

4. 200mL fermentation.

5. Protein purification (1-step affinity purification).

6. WB and SDS-PAGE test.

7. QC and report.

1. 0.5mg-5mg purified protein.

2. Data and QC report.

Inquiry​

P110313

Yeast-Comfo

7 – 9 Weeks

1. Condon optimization and gene synthesis (free of charge).

2. Minimum 2 expression strains or vectors will be tested: transformation and high expression monoclone selection.

3. Expression condition test: up to 84 different conditions.

4. 200mL - 1L fermentation.

5. Protein purification with 2 steps, purity polishing to >90%.

6. WB and SDS-PAGE test.

7. QC and report.

8. One time video meeting.

1. 1mg - 20mg purified protein.

2. Data and QC report.

Inquiry​

P110302

1L fermentation

3~5 days

1L fermentation and protein purification for 1 clone.

Up to 500mg purified protein.

Inquiry​

P110303

5L fermentation

5~7 days

5L fermentation and protein purification for 1 clone.

Up to 2500mg purified protein.

Inquiry​

 

Optional Service
1. Tag remove
2. 10L~4000L fermentation
3. Western Blot for protein confirmation
4. Mass Spectrometry
More information: Please Email to info@bio-bench.com if you need special service Yeast Expression System.

Comparison of different types of service

                                  Service type

Content

Yeast

Ready

Yeast

StepUp

Yeast

Comfo

Codon optimization

-

✓

✓

Gene synthesis

-

✓

✓

Multiple vector or multiple strain test

-

-

✓

Expression temperature test: HighT °C

✓

✓

✓

Expression temperature test: LowT °C

-

-

✓

Expression duration test (time points)

1

3

5 - 7

Multiple concentration of inducing reagent

1

2

2

Fermentation volume

80mL

200mL

200mL – 1L

Affinity purification

✓

✓

✓

2nd purification: purity enhancement

-

-

✓

SDS-PAGE analysis

✓

✓

✓

WB analysis

-

✓

✓

Data, QC and report

✓

✓

✓

Video meeting support

-

-

✓

Purified protein

0.3mg – 2mg

0.5mg – 5mg

1mg – 10mg

 

Inquiry​

Inquiry​

Inquiry​

 

List of Vectors
pPIC9k, pPIC3.5, pPICZαA, pAO815, pYES2/NTA. Please inquire for the up-to-date list.
List of Expression Strain
GS115, KM71, X33, INVSC1. Please inquire for the up-to-date list.
bio bench your reliable cro partner aaalac gmp

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Hybridoma mAb
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​

Protein

E. coli
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Yeast
Insect Cell
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​Protein Production
​
Protein Engineering

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  • Antibody
    • Polyclonal Ab
    • Hybridoma mAb
    • Phage Display mAb
    • Human Antibody
    • Antibody Engineering
  • Protein
    • E.coli
    • B.Subtilis
    • Yeast
    • Insect Cell
    • Mammalian
    • Antibody Production
    • Stable Cell Line
  • Drug Discovery
  • Assay Kit Service
  • Catalog Product
    • AccuPAGE™ Bis-Tris Precast PAGE Gel
    • AccuPAGE™ Tris-Glycine Precast PAGE Gel
    • COVID-19
    • Covid-19 Kit
  • Support
    • Buffer and Reagent
    • Knowledge base
  • Contact Us
    • Company
    • Career
    • Event