Service
and Timeline |
Service
Description |
Deliverables |
Price |
P110411 Mammalian-Ready 4 – 6 Weeks |
1. Cell transfection. 2. 80mL cell culture. 3. Expression condition test: up to
2 different conditions. 4. Protein purification (1-step
affinity purification). 5. SDS-PAGE test. 6. QC and report. |
1. 0.3mg-2mg purified protein. 2. Data and QC report. |
|
P110412 Mammalian-StepUp 8 - 12 Weeks |
1.
Condon optimization and gene synthesis (1000bp free). 2.
Cell transfection. 3.
Expression condition test: up to 3 different conditions. 4.
200mL fermentation. 5.
Protein purification (1-step affinity purification). 6. WB
and SDS-PAGE test. 7. QC
and report. |
1. 0.5mg-5mg purified protein. 2. Data and QC report. |
|
P110413 Mammalian-Comfo 9 - 13 Weeks |
1.
Condon optimization and gene synthesis (free of charge). 2. 2
expression vectors will be tested. 3. Transfection condition test: 6
conditions. Cell transfection. 4. Expression condition test: up to
3 different conditions. 5. 200mL - 1L fermentation. 6. Protein purification with 2
steps, purity polishing to >90%. 7. WB and SDS-PAGE test. 8. QC and report. 9. One time video meeting. |
1. 1mg - 10mg purified protein. 2. Data and QC report. |
Optional Service 1. Tag remove 2. 5L~200L fermentation, serum free 3. Western Blot for protein confirmation 4. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service for Mammalian Cell Expression System. |
Service type Content |
Mammalian Ready |
Mammalian StepUp |
Mammalian Comfo |
Codon
optimization |
- |
✓ |
✓ |
Gene
synthesis |
- |
✓ |
✓ |
Multiple
vector or multiple cell line test |
- |
- |
✓ |
Expression
temperature test: HighT °C |
✓ |
✓ |
✓ |
DNA
to lipid ratio test |
- |
- |
✓ |
Expression
duration test (time points) |
1 |
3 |
5 |
Fermentation
volume |
80mL |
200mL |
200mL – 1L |
Affinity
purification |
✓ |
✓ |
✓ |
2nd
purification: purity enhancement |
- |
- |
✓ |
SDS-PAGE
analysis |
✓ |
✓ |
✓ |
WB
analysis |
- |
✓ |
✓ |
Data,
QC and report |
✓ |
✓ |
✓ |
Video
meeting support |
- |
- |
✓ |
Purified
protein |
0.3mg – 2mg |
0.5mg – 5mg |
1mg – 10mg |
|
Service and Timeline |
Client Provides |
Service Description |
Deliverables |
Price |
P110416 Guaranteed Mammalian Expression
service 5 – 8 weeks* |
1. Reconstructed plasmid. If client
failed to provide the plasmid, Bio Bench will synthesize it according to
client provided cDNA sequence under minimum cost. 2. Previous test results:
expression level (mg/L) together with credited data (SDS-PAGE, WB, ELISA, etc.). 3. Purification protocol. 4. Or any type of open source
information that show the expression and purification of the protein. |
1. Plasmid maxi-prep. 2. CHO/HEK293 cell culture
preparation. 3. Cell transfection and cell
culture. 4. Protein purification through
combined methods. Protein lyophilisation. 5. QC: SDS-PAGE, WB and/or ELISA
according to the protein. |
1. 3mg – 5mg purified protein,
lyophilized or liquid form. 2. Protein purity: > 80%. 3. QC and Report. |
PROMO! |
P110416 |
Other quantity also available! 5 –
10mg, 10 – 20mg, 20 – 30mg, 30 – 40mg and 40 – 50mg. Please inquire Bio Bench for the detail. |
List of Vectors |
Commercial vectors and Bio Bench improved vectors. Please inquire for the up-to-date list. |
List of Expression Strain |
CHO-K1, HEK293T. Please inquire for the up-to-date list. |
Mammalian-ST™ is the stable mammalian cell line development service provided by Bio Bench. Bio Bench has achieved more than 600 stable cell line projects since 2013, including more than 50 antibody drug expressing cell lines. The table below lists the cell types that have been successfully generated into stable cell line, do not hesitate to request a quote from us!
Stable mammalian cell line is one of the most important tools in drug discovery, toxicity test, biological research and bio-pharma industry. In order to establish stable cell line capable of long-term stably expressing the gene of interest (GOI), the operation is commonly achieved by transfection of a reconstructed plasmid containing the GOI together with a selection marker (antibiotic or fluorescent protein), through either liposome-mediated transfection or virus infection (or called viral transduction).
|
|
Bio Bench has successfully established the following
host into stable cell line |
|||
CHO |
CHO-K1 |
expiCHO |
CHO-S |
293F |
CHO-DG44 |
HEK293 |
293T |
expi293F |
Vero (insect cell) |
Your cell line here! Get
a quote for free |
Service type Specification |
Transient transfection |
Stable pool |
Stable cell line |
Initial cost |
+ |
++ |
+++ |
Midi scale cell culture or production cost |
+++ |
++ |
++ |
Large scale cell culture or production cost |
+++++ |
++ |
+ |
Operation complexity at small scale |
++ |
+ |
+ |
Operation
complexity at midi scale |
+++ |
+ |
+ |
Operation complexity at large scale |
+++++ |
+ |
+ |
Operator-to-operator variation |
++++ |
+ |
+ |
Batch-to-batch variation (by the same operator) |
++ |
+ |
+ |
Protein expression level |
+ |
+++ |
+++++ |
Short term expression stability (1 - 3 days) |
++++ |
+++++ |
+++++ |
Long term
expression stability (10 – 20 days) |
\ |
++ |
+++++ |
Regeneration stability |
\ |
++ |
+++++ |
Conclusion |
1. Cell culture volume <100mL, or occasionally used cell culture. 2. Protein amount <10mg, or occasionally used protein. 3. Transfection is request by experiment protocol. 4. Co-transfection of more than 1 plasmid. 5. Small scale and
proof-of-concept experiment. |
1. 100mL < Cell culture volume < 20L, or regularly used cell
culture (without specific requirement). 2. 10mg < Protein amount < 5g, or one time production but enough
for a long period of usage. 3. Non-commercial purpose, or less sensitive to cost. 4. Low/no requirement for cell homogeneity. 5. Low requirement
for stability. |
1. Small to high volume cell culture, with or
without high requirement. 2. Small to high amount of protein production. 3. Commercial purpose, cost effective. 4. Experiment requires high level of cell
homogeneity. 5. High requirement for cell stability. |
|
Service and Timeline |
Service Description (To be determined according to your specific
project) |
Deliverables |
Price |
P1521 – Stable pool Mammalian stable pool generation
service 8 – 10 Weeks |
1. Positive control
protein/antibody characterization. 2. Codon optimization, gene
synthesis, cloning and plasmid preparation. 3. Transient transfection test and
antibiotic concentration determination. 4. Transfection and stable pool
generation. 5. Stable pool characterization:
expression level, cell viability, growth curve and total cell count. More
characteristics optional! 6. SDS-PAGE, WB, ELISA, and/or FACS
analysis. 7. Protein sample expression and
purification. 8. QC and report. |
- Selected and optimized stable
pool. - Purified protein sample. - Data and QC report. |
|
P1526 – Stable cell line Mammalian stable cell line
generation service 23 – 30 Weeks |
All of the content from P1521
Stable pool generation service, together with the following: 1. Monoclonalization
and best clones selection. 2. Fed-batch 1 (small culture
volume) primary study and characterization. At least 6 characteristics will
be tested for a duration of 8 – 15 days. 3. Fed-batch 2 (midi culture
volume) enhanced study and characterization, optional. 4. Cell banking. 5. Whole genome sequencing of the
selected clone, and backup plasmid establishment. |
- Selected stable cell line. - Purified protein sample. - Data and QC report. |
|
More
test options, including: 1. Bioactivity test: binding/blocking
assay, ADCC assay, etc. 2. Affinity test by biacore or ForteBio method. 3. Cell culture monitoring: Ammonia
test, lactic acid test, pCO2 analysis, etc. 4. Charge heterogeneity test. 5. MS test: molecular weight,
aggregation, etc. Would like to lean more? Seeking a
specific testing method? Click on Inquiry and we will get back to you soon. |