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Mammalian Cell Expression system is able to perform all of the post translational modifications to the protein. Therefore mammalian cell line is not only used for protein production, but also for immunogen, experiment material and down-stream validation.
Service highlight: 1. Team members have average 10 years’ experience in mammalian expression system. 2. Up to 200L serum-free fermentation system. 3. 90% success rate for protein expression. |
Service and Time Line |
Service Description |
Deliverables |
Price |
P110500 Standard Mammalian Transient cell line (HEK293 / CHO) 8~13 weeks |
1. Condon optimization and gene synthesis. 2. Transfection and cell culture. 3. Expression test (plasmid extraction, transformation, SDS analysis, WB analysis, 80mL fermentation and purification) 4. QC and report. |
•Transient cell line. •1 reconstructed plasmid. •0.5-3mg protein. •QC and report. |
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P110501 Stable Cell line 30~40 days |
1 stable expression cell line will be selected. |
•Transient cell line. •0.5-3mg protein. •QC and report. |
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P110502 HEK293 1L fermentation 1~2 weeks |
HEK293 cell line 1L fermentation and protein purification for 1 clone. |
•1mg~30mg protein. |
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P110503 CHO 1L fermentation 1~2 weeks |
CHO cell line 1L fermentation and protein purification for 1 clone. |
•1mg~30mg protein. |
Optional Service 1. Tag remove 2. 5L~200L fermentation, serum free 3. Western Blot for protein confirmation 4. Mass Spectrometry More information: Please Email to info@bio-bench.com if you need special service for Mammalian Cell Expression System. |
List of Vectors |
Commercial vectors and Bio Bench improved vectors. Please inquire for the up-to-date list. |
List of Expression Strain |
CHO-K1, HEK293T. Please inquire for the up-to-date list. |
