Protease |
Cleavage site |
Location
and residue |
pH |
Inhibitor |
Salt sensitivity |
Chaotrope
sensitivity |
Enzyme-to-protein
ratio |
Endoprotease |
|||||||
Enteropeptidase (EK) |
DDDDK↓ |
N-, none C-, DDDDK |
5.5 – 8.5 |
Reducing
agents |
|
|
0.1% (wt/wt) |
Thrombin |
LVPR↓GS |
N-, GS C-, LVPR |
6 -
9 |
Reducing agents |
≤ 0.15M |
≤ 0.1M urea |
1 – 10% (wt/wt) |
Factor Xa |
LVPR↓GS |
N-,
none C-,
IEGR |
6 -
9 |
Reducing agents,
chelating agents, phosphate ions. |
≤ 0.15M |
≤ 0.1M urea |
1 – 10% (wt/wt) |
TEV Protease |
ENLYFQ↓(G/S) |
N-,
G/S C-,
ENLYFQ |
5.5
– 8.5 |
Thiol alkylating agents |
≤ 0.1M |
2M
urea |
1 –
3% (wt/wt) |
Rhinovirus 3C Protease |
LEVLFQ↓GP |
N-,
GP C-,
LEVLFQ |
5.5 – 8.5 |
Thiol alkylating agents |
|
|
|
SUMO Protease |
SUMO↓X |
N-
only, no residue |
6 -
9 |
|
≤0.5M |
≤ 0.1M GnCl ≤ 2M urea |
0.1% (wt/wt) |
Immoblized
subtilisin (BPN) |
Propeptide↓X |
N- only, no residue |
7.2 |
|
|
≤ 4M urea |
1:1 (mol/mol) |
Exoprotease |
|||||||
Carboxypeptidase
A |
|
C-terminal
amino acids except Pro, Lys and Arg. |
|
Reducing
agents, chelating
agents |
|
|
|
Carboxypeptidase B |
|
C-terminal Lys and Arg. |
|
Reducing agents, chelating agents |
|
|
|
DAPase |
|
N-terminal dipeptides |
|
Reducing agents, thiol alkylating agents. |
|
|
|