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Back to Buffer and Reagent

Phosphate-Buffered Saline with Tween 20 (10x stock, 1L)

Alias: PBST

Reagent

Amount

Final Concentration

NaCl

80 g

1.37 M

KCl

2.0 g

27 mM

Na2HPO4

14.4 g

100 mM

KH2PO4

2.4 g

18 mM

Tween-20

20 mL

2%

Directions

Mix all of the above with 800mL ultra-pure water and dissolve well till no particle observed.

Adjust pH to 7.2, 7.4 or 7.6 with 5M HCl.

Then add ultra-pure water till 1L.

Sterilization

PBS can be sterilized by autoclaving for 20 min at 121°C or 15 psi (1.05 kg/cm2).

PBST may be filter with 0.22um membrane filter.

TWEEN-20 is not stable to autoclaving, particularly with metal cations in buffer solutions. TWEEN-20 is heat sensitive and will darken when exposed to elevated temperatures.

Usage

Immunology assay, WB

Storage

Room temperature, avoid direct sunshine.

 

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  • Research Services
    • Gene Synthesis
    • Peptide Synthesis
    • Antibody
      • Polyclonal Ab
      • Hybridoma mAb
      • Rabbit mAb
      • Phage Display mAb
      • Human Antibody
      • Antibody Engineering
    • Protein
      • E.coli
      • B.Subtilis
      • Yeast
      • Insect Cell
      • Mammalian
      • Antibody Production
      • Stable Cell Line
  • Antibody Drug Discovery
  • Catalog Product
    • Pathogen
      • hMPV
      • Mpox
    • AccuPAGE™ Precast Gel
      • AccuPAGE™ Bis-Tris Precast PAGE Gel
      • AccuPAGE™ Tris-Glycine Precast PAGE Gel
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