Phosphate-Buffered Saline with Tween 20 (1x working
solution, 1L) |
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Alias: PBST |
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Reagent |
Amount |
Final Concentration |
NaCl |
8g |
137 mM |
KCl |
0.2g |
2.7 mM |
Na2HPO4 |
1.44g |
10 mM |
KH2PO4 |
0.24g |
1.8 mM |
Tween 20 |
2mL |
0.2% |
Directions Mix
all of the above with 800mL ultra-pure water and dissolve well till no particle
observed. Adjust
pH to 7.2, 7.4 or 7.6 with 5M HCl. Then
add ultra-pure water till 1L. |
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Sterilization PBS
can be sterilized by autoclaving for 20 min at 121°C or 15 psi (1.05 kg/cm2). PBST
may be filter with 0.22um membrane filter. TWEEN
20 is not be stable to autoclaving, particularly with metal cations in buffer
solutions. TWEEN 20 is heat sensitive and will darken when exposed to
elevated temperatures. |
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Usage Immunology
assay, WB |
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Storage Room
temperature, avoid direct sunshine. |