Phosphate Buffer pH5.8 to 8.0 | BIO BENCH - BIO BENCH

Phosphate Buffer (0.1M, 1x working solution, pH from 5.8 to 8.0 at 25°C)
Alias: \
pH at 25°C	Solution A (mL) 0.2M Na2HPO4	Solution B (mL) 0.2M NaH2PO4
5.8	40	460
6.0	61.5	438.5
6.2	92.5	407.5
6.4	132.5	367.5
6.6	187.5	312.5
6.8	245	255
7.0	305	195
7.2	360	140
7.4	405	95
7.6	435	65
7.8	457.5	42.5
8.0	473.5	26.5
Directions Solution A: 0.2M sodium phosphate, dibasic dehydrate (Na2HPO4•2H2O) Add 35.598g Na2HPO4•2H2O into 800mL ultra-pure water and dissolve well till no particle observed. Then add ultra-pure water till 1L. Solution B: 0.2M sodium phosphate, monobasic, monohydrate (NaH2PO4•H2O). Add 27.598g NaH2PO4•H2O into 800mL ultra-pure water and dissolve well till no particle observed. Then add ultra-pure water till 1L. Mix Solution A and Solution B at indicated volume, according to your desired pH. Test the pH and adjust it with 5M HCl or 5M NaOH, if necessary. Then add ultra-pure water till 1L.
Sterilization Filter with 0.22um membrane filter. Sterilized by autoclaving for 20 min at 121°C or 15 psi (1.05 kg/cm2).
Usage Cell study, Immunology assay, Molecular biology, Protein analysis, Physiology test, Histology test
Storage Room temperature, avoid direct sunshine.