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Back to Buffer and Reagent

Phosphate Buffer (0.1M, 1x working solution, pH from 5.8 to 8.0 at 25°C)

Alias: \

pH at 25°C

Solution A (mL)

0.2M Na2HPO4

Solution B (mL)

0.2M NaH2PO4

5.8

40

460

6.0

61.5

438.5

6.2

92.5

407.5

6.4

132.5

367.5

6.6

187.5

312.5

6.8

245

255

7.0

305

195

7.2

360

140

7.4

405

95

7.6

435

65

7.8

457.5

42.5

8.0

473.5

26.5

Directions

Solution A: 0.2M sodium phosphate, dibasic dehydrate (Na2HPO4•2H2O) Add 35.598g Na2HPO4•2H2O into 800mL ultra-pure water and dissolve well till no particle observed. Then add ultra-pure water till 1L.

Solution B: 0.2M sodium phosphate, monobasic, monohydrate (NaH2PO4•H2O). Add 27.598g NaH2PO4•H2O into 800mL ultra-pure water and dissolve well till no particle observed. Then add ultra-pure water till 1L.

Mix Solution A and Solution B at indicated volume, according to your desired pH.

Test the pH and adjust it with 5M HCl or 5M NaOH, if necessary.

Then add ultra-pure water till 1L.

Sterilization

Filter with 0.22um membrane filter.

Sterilized by autoclaving for 20 min at 121°C or 15 psi (1.05 kg/cm2).

Usage

Cell study, Immunology assay, Molecular biology, Protein analysis, Physiology test, Histology test

Storage

Room temperature, avoid direct sunshine.

 

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  • Antibody
    • Polyclonal Ab
    • Hybridoma mAb
    • Phage Display mAb
    • Human Antibody
    • Antibody Engineering
  • Protein
    • E.coli
    • B.Subtilis
    • Yeast
    • Insect Cell
    • Mammalian
    • Antibody Production
    • Stable Cell Line
  • Drug Discovery
  • Assay Kit Service
  • Catalog Product
    • AccuPAGE™ Bis-Tris Precast PAGE Gel
    • AccuPAGE™ Tris-Glycine Precast PAGE Gel
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  • Support
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