- AccuPAGE™ Precast Gel - Protein Electrophoresis
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- AccuPAGE™ Tris-Glycine Precast PAGE Gel
AccuPAGE™ Tris-Glycine Precast PAGE Gel
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https://www.helixarbio.com/products/accupage-tris-glycine-precast-gel
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Bio Bench AccuPAGE™ protein electrophoresis gels are precast polyacrylamide gels that provide large range of molecular weight separation ability with good resolution and sample integrity. AccuPAGE™ provides 2 types of gels, including conventional Tris-Glycine gel and the Bis-Tris gel. These 2 types of gel are both optimized by Bio Bench, in order to provide better performance as well as good stability during long term storage. AccuPAGE™ is compatible with nearly all of tanks from major biotech providers. Take out from the fridge and open the package and the sealing, then you can operate it under the routine lab instructions.
AccuPAGE™ Tris-Gly gel is made of optimized formula from conventional Tris-Glycine gel. It has good stability and long shelf life for up to 12 months.
AccuPAGE™ Bis-Tris gel delivers consistent gel performance with a neutral pH environment (pH≈7.0) that minimizes any protein modifications. Bis-Tris gel can only run in MOPS or MES buffer. Please feel relaxed to use Bis-Tris gel - If your lab is already running Tris-Glycine gel, then it is fully compatible to run your sample in AccuPAGE™ Bis-Tris gel! Bis-Tris can improve the stability of the gel structure during electrophoresis, which will avoid the gel become trapezoid shape and lead to sharp and straight band after staining.
AccuPAGE™ gel separation
How to use the AccuPAGE™ precast gel - a quick guide
AccuPAGE™ precast gel family
Gel type |
Running Buffer |
Gel Thickness |
Gel Concentration |
Max Separation |
Recommended Separation |
Well Amount |
Max Sample Load |
Cat. No. |
Tris-Glycine |
Tris-Glycine |
1mm |
6% |
40-300kDa |
60-200kDa |
10 |
50μL |
|
15 |
30μL |
|||||||
8% |
25-300kDa |
40-200kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
10% |
11-230kDa |
20-160kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
12% |
6.5-200kDa |
15-85kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
15% |
5-170kDa |
10-50kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
4% - 15% |
5-270kDa |
20-200kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
4% - 20% |
3.5-300kDa |
5-200kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
Tris-Bis |
MOPS or MES |
1mm |
8% |
8-270kDa |
25-245kDa |
10 |
50μL |
|
15 |
30μL |
|||||||
10% |
8-270kDa |
11-180kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
12% |
3.5-245kDa |
11-135kDa |
10 |
50μL |
||||
15 |
30μL |
|||||||
4% - 12% |
3.5-270kDa |
11-245kDa |
10 |
50μL |
||||
15 |
30μL |
Accessories for AccuPAGE™ precast gel
Product Name |
Description |
Cat.No. |
Tank Adapter Plate – Type 1 |
Tank
Adapter designed for the following models: 1.
Sigma
Aldrich Dual Run 2.
Thermofisher/Invitrogen XCell (I, II,
& Surelock™ mini-cell) 3.
LONZA PAGEr® Minigel Chamber |
|
Tank Adapter Accessory – Type 2 |
Tank
Adapter designed for the following model: 1.
Life
Technologies Mini Gel Tank |
|
Ultra Fast Running Buffer for Tris-Gly Gel |
Ultra
Fast Running Buffer designed for Tris-Gly gel, running time as little as 20min. 1 pack for
500mL running buffer. |
AP-B-P500 Request
for purchase. |
AccuPAGE™ precast gel specifications
Specifications |
Tris-Glycine |
Bis-Tris |
Concentration |
6%, 8%, 10%, 12%,
15%, 4-15%, 4-20% |
8%, 10%, 12%, 4-12% |
Gel Dimension |
Mini gel,
84mm(W)×74mm(H) ×1mm(D) |
|
Cassette Dimension |
100mm(W)×89mm(H) ×4.8mm(D) |
|
Gel Thickness |
1mm |
|
Stacking Gel |
Concentration 4%,
15mm(H) |
|
Well amount and max load |
10 wells 50μL, or 15 wells 30μL. |
|
Storage and Shelf Life |
4 - 8°C, up to 12
months. DO NOT FREEZE. |
4 - 8°C, up to 12
months. DO NOT FREEZE. |
Shipping |
Room temperature or
controlled temperature. |
|
Tank Compatibility |
AccuPAGE™ is designed to be
compatible with all types of MINI-PAGE tank which allows 10cm width gel
cassette. Including but not limited to the following models:
|
|
Running Time |
Minimum 20min if Ultra Fast Running buffer is used. 40 – 60 min by
using common buffer. |
|
Application |
Native PAGE,
denaturing PAGE. Note: Gel does NOT
contain detergent (like SDS). |
|
Running Buffer |
Tris-Glycine |
MOPS or MES |
Package Quantity |
2 pcs test pack, 10
pcs, 30 pcs and 50 pcs. |
|
* Accessory or
adaption will be needed. Bio Bench provides the accessories. |