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AccuPAGE™ Tris-Glycine Precast PAGE Gel

SKU:
€29.00
29 495 €29.00 - €495.00
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per item
Contact us for purchase or request sample: [email protected]

Documents

Datasheet - AccuPAGE™ Precast Gel
User Guide - AccuPAGE™ Precast Gel

Bio Bench AccuPAGE™ protein electrophoresis gels are precast polyacrylamide gels that provide large range of molecular weight separation ability with good resolution and sample integrity. AccuPAGE™ provides 2 types of gels, including conventional Tris-Glycine gel and the Bis-Tris gel. These 2 types of gel are both optimized by Bio Bench, in order to provide better performance as well as good stability during long term storage. AccuPAGE™ is compatible with nearly all of tanks from major biotech providers. Take out from the fridge and open the package and the sealing, then you can operate it under the routine lab instructions.

AccuPAGE™ Tris-Gly gel is made of optimized formula from conventional Tris-Glycine gel. It has good stability and long shelf life for up to 12 months.

AccuPAGE™ Bis-Tris gel delivers consistent gel performance with a neutral pH environment (pH≈7.0) that minimizes any protein modifications. Bis-Tris gel can only run in MOPS or MES buffer. Please feel relaxed to use Bis-Tris gel - If your lab is already running Tris-Glycine gel, then it is fully compatible to run your sample in AccuPAGE™ Bis-Tris gel! Bis-Tris can improve the stability of the gel structure during electrophoresis, which will avoid the gel become trapezoid shape and lead to sharp and straight band after staining.

AccuPAGE™ gel separation

How to use the AccuPAGE™ precast gel - a quick guide

AccuPAGE™ precast gel family

Gel type

Running Buffer

Gel Thickness

Gel Concentration

Max Separation

Recommended Separation

Well Amount

Max Sample Load

Cat. No.

Tris-Glycine

Tris-Glycine

1mm

6%

40-300kDa

60-200kDa

10

50μL

AP-GPG-610

15

30μL

AP-GPG-615

8%

25-300kDa

40-200kDa

10

50μL

AP-GPG-810

15

30μL

AP-GPG-815

10%

11-230kDa

20-160kDa

10

50μL

AP-GPG-1010

15

30μL

AP-GPG-1015

12%

6.5-200kDa

15-85kDa

10

50μL

AP-GPG-1210

15

30μL

AP-GPG-1215

15%

5-170kDa

10-50kDa

10

50μL

AP-GPG-1510

15

30μL

AP-GPG-1515

4% - 15%

5-270kDa

20-200kDa

10

50μL

AP-GPG-41510

15

30μL

AP-GPG-41515

4% - 20%

3.5-300kDa

5-200kDa

10

50μL

AP-GPG-42010

15

30μL

AP-GPG-42015

Tris-Bis

MOPS or MES

1mm

8%

8-270kDa

25-245kDa

10

50μL

AP-GPB-810

15

30μL

AP-GPB-815

10%

8-270kDa

11-180kDa

10

50μL

AP-GPB-1010

15

30μL

AP-GPB-1015

12%

3.5-245kDa

11-135kDa

10

50μL

AP-GPB-1210

15

30μL

AP-GPB-1215

4% - 12%

3.5-270kDa

11-245kDa

10

50μL

AP-GPB-41210

15

30μL

AP-GPB-41215

 

Accessories for AccuPAGE™ precast gel

Product Name

Description

Cat.No.

Tank Adapter Plate – Type 1

Tank Adapter designed for the following models:

1.       Sigma Aldrich Dual Run

2.       Thermofisher/Invitrogen XCell (I, II, & Surelock™ mini-cell)

3.       LONZA PAGEr® Minigel Chamber

AP-AC-01

Tank Adapter Accessory – Type 2

Tank Adapter designed for the following model:

1.       Life Technologies Mini Gel Tank

AP-AC-02

Ultra Fast Running Buffer for Tris-Gly Gel

Ultra Fast Running Buffer designed for Tris-Gly gel, running time as little as 20min. 1 pack for 500mL running buffer.

AP-B-P500

Request for purchase.

 

AccuPAGE™ precast gel specifications

Specifications

Tris-Glycine

Bis-Tris

Concentration

6%, 8%, 10%, 12%, 15%, 4-15%, 4-20%

8%, 10%, 12%, 4-12%

Gel Dimension

Mini gel, 84mm(W)×74mm(H) ×1mm(D)

Cassette Dimension

100mm(W)×89mm(H) ×4.8mm(D)

Gel Thickness

1mm

Stacking Gel

Concentration 4%, 15mm(H)

Well amount and max load

10 wells 50μL, or 15 wells 30μL.

Storage and Shelf Life

4 - 8°C, up to 12 months.

DO NOT FREEZE.

4 - 8°C, up to 12 months.

DO NOT FREEZE.

Shipping

Room temperature or controlled temperature.

Tank Compatibility

AccuPAGE™ is designed to be compatible with all types of MINI-PAGE tank which allows 10cm width gel cassette. Including but not limited to the following models:

  1. Bio-Rad Mini-PROTEAN (II/3 /Tetra System) *
  2. Hoefer™ Mighty Small (SE250 / SE260 / SE280)
  3. Sigma Aldrich Dual Run *
  4. Thermofisher/Invitrogen XCell (I, II, & Surelock™ mini-cell) *
  5. LONZA PAGEr® Minigel Chamber *
  6. Tanon

Running Time

Minimum 20min if Ultra Fast Running buffer is used.

40 – 60 min by using common buffer.

Application

Native PAGE, denaturing PAGE.

Note: Gel does NOT contain detergent (like SDS).

Running Buffer

Tris-Glycine

MOPS or MES

Package Quantity

2 pcs test pack, 10 pcs, 30 pcs and 50 pcs.

* Accessory or adaption will be needed. Bio Bench provides the accessories.