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Phosphate-Buffered
Saline (PBS, 10x stock, 1L) |
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Reagent |
Amount |
Final Concentration |
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NaCl |
80g |
1.37 M |
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KCl |
2.0g |
27 mM |
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Na2HPO4 |
14.4g |
100 mM |
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KH2PO4 |
2.4g |
18 mM |
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If necessary, PBS may be supplemented with the
following: |
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CaCl2•2H2O |
1.33 g |
10 mM |
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MgCl2•6H2O |
1.0 g |
5 mM |
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Direction Mix all of the above with 800mL ultra-pure
water and dissolve well till no particle observed. Adjust pH to 7.2, 7.4 or 7.6 with pure HCl. Then add ultra-pure water till 1L. |
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Sterilization Filter with 0.22um membrane filter. Or sterilize them by autoclaving for 20 min
at 121°C or 15 psi (1.05 kg/cm2) |
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Usage General biology experiment, cell
experiment, immunology experiment. |
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Storage Room temperature, avoid direct sunshine. |
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