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Buffer and Reagent​

Back to Buffer and Reagent

Phosphate-Buffered Saline (PBS, 10x stock, 1L)

Reagent

Amount

Final Concentration

NaCl

80g

1.37 M

KCl

2.0g

27 mM

Na2HPO4

14.4g

100 mM

KH2PO4

2.4g

18 mM

If necessary, PBS may be supplemented with the following:

CaCl2•2H2O

1.33 g

10 mM

MgCl2•6H2O

 1.0 g

5 mM

Direction

Mix all of the above with 800mL ultra-pure water and dissolve well till no particle observed.

Adjust pH to 7.2, 7.4 or 7.6 with pure HCl.

Then add ultra-pure water till 1L.

Sterilization

Filter with 0.22um membrane filter.

Or sterilize them by autoclaving for 20 min at 121°C or 15 psi (1.05 kg/cm2)

Usage

General biology experiment, cell experiment, immunology experiment.

Storage

Room temperature, avoid direct sunshine.

 

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  • Antibody
    • Polyclonal Ab
    • Hybridoma mAb
    • Phage Display mAb
    • Human Antibody
    • Antibody Engineering
  • Protein
    • E.coli
    • B.Subtilis
    • Yeast
    • Insect Cell
    • Mammalian
    • Antibody Production
    • Stable Cell Line
  • Drug Discovery
  • Assay Kit Service
  • Catalog Product
    • AccuPAGE™ Bis-Tris Precast PAGE Gel
    • AccuPAGE™ Tris-Glycine Precast PAGE Gel
    • COVID-19
    • Covid-19 Kit
  • Support
    • Buffer and Reagent
    • Knowledge base
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