Tag |
Size |
Tag placement |
Uses |
Typical
sequence |
Affinity tags |
||||
His tag |
6 or 8 aa |
N-, C-, internal |
Purification |
HHHHHH |
Strep II tag |
8 aa |
Purification |
WSHPQFEK |
|
Avi tag |
15 aa |
N-, C- |
Purification |
GLNDIFEAQKIEWHE |
T7 tag |
11 or 16 aa |
N-, internal |
Purification |
MASMTGGQQMG |
FLAG tag |
8 aa |
N-, C- |
Purification |
DYKDDDDK |
S tag |
15 aa |
N-, C- |
Purification |
KETAAAKFERQHMDS |
HA tag |
9 aa |
N-, C- |
Purification |
YPYDVPDYA |
C-Myc tag |
11 aa |
N-, C- |
Purification |
EQKLISEEDL |
Chitin binding domain |
51 aa |
N-, C- |
Purification |
|
Calmodulin binding domain |
26 aa |
N-, C- |
Purification |
KRRWKKNFIAVSAANRFKKISSSGAL |
Cellulose binding domain |
27 – 129 aa |
N-, C- |
Purification |
TQSHYGQCGGIGYSGPTVCASGTTCQVLNPYYSQCL[1] |
Solubility-enhancing
tag |
||||
GST |
211 aa (26 kDa) |
N- |
Enhancing solubility & purification |
|
MBP |
396 aa (40 kDa) |
N-, C- |
Enhancing solubility & purification |
|
T7 gene 10 |
260 aa |
N- |
Enhancing solubility & purification |
|
NusA |
495 aa (54.8 kDa) |
N- |
Enhancing solubility |
|
Thioredoxin (Trx tag) |
109 aa |
N-, C- |
Enhancing solubility |
|
SUMO |
100 aa |
N- |
Enhancing solubility |
|
Ubiquitin |
76 aa |
N- |
Enhancing solubility |